Scientific studies

STUDIES

Li, J., et al., Protective effects of molecular hydrogen on steroid-induced osteonecrosis in rabbits via reducing oxidative stress and apoptosis. BMC Musculoskelet Disord, 2017. 18(1): p. 58.
Guo, J., et al., Hydrogen-rich saline prevents bone loss in diabetic rats induced by streptozotocin. Int Orthop, 2017. 41(10): p. 2119-2128.
Yamada, T., et al., Hydrogen supplementation of preservation solution improves viability of osteochondral grafts. ScientificWorldJournal, 2014. 2014: p. 109876.
Wan, W.L., et al., An In Situ Depot for Continuous Evolution of Gaseous H2 Mediated by a Magnesium Passivation/Activation Cycle for Treating Osteoarthritis. Angew Chem Int Ed Engl, 2018.
Ostojic, S.M., et al., Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries. Postgrad Med, 2014. 126(5): p. 187-95.
Sun, Y., et al., Treatment of hydrogen molecule abates oxidative stress and alleviates bone loss induced by modeled microgravity in rats. Osteoporos Int, 2013. 24(3): p. 969-78.
Li, D.Z., et al., Treatment with hydrogen molecules prevents RANKL-induced osteoclast differentiation associated with inhibition of ROS formation and inactivation of MAPK, AKT and NF-kappa B pathways in murine RAW264.7 cells. J Bone Miner Metab, 2013.
Guo, J.D., et al., Hydrogen water consumption prevents osteopenia in ovariectomized rats. Br J Pharmacol, 2013. 168(6): p. 1412-20.
Cai, W.W., et al., Treatment with hydrogen molecule alleviates TNFalpha-induced cell injury in osteoblast. Mol Cell Biochem, 2013. 373(1-2): p. 1-9.
Xu, Z., et al., Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema. J Inflamm (Lond), 2012. 9: p. 2.
Takeuchi, S., et al., Hydrogen may inhibit collagen-induced platelet aggregation: an ex vivo and in vivo study. Internal Medicine, 2012. 51(11): p. 1309-13.
Lekic, T., et al., Protective effect of hydrogen gas therapy after germinal matrix hemorrhage in neonatal rats.. Acta Neurochir Suppl, 2011. 111: p. 237-41.
Kubota, M., et al., Hydrogen and N-acetyl-L-cysteine rescue oxidative stress-induced angiogenesis in a mouse corneal alkali-burn model. Investigative Ophthalmology and Visual Science, 2011. 52(1): p. 427-33.
Itoh, T., et al., Molecular hydrogen inhibits lipopolysaccharide/interferon gamma-induced nitric oxide production through modulation of signal transduction in macrophages. Biochemical and Biophysical Research Communications, 2011. 411(1): p. 143-9.
Hanaoka, T., et al., Molecular hydrogen protects chondrocytes from oxidative stress and indirectly alters gene expressions through reducing peroxynitrite derived from nitric oxide. Medical Gas Research, 2011. 1(1): p. 18.
Kawasaki, H., J.J. Guan, and K. Tamama, Hydrogen gas treatment prolongs replicative lifespan of bone marrow multipotential stromal cells in vitro while preserving differentiation and paracrine potentials. Biochemical and Biophysical Research Communications, 2010. 397(3): p. 608-613.

Wu, J., et al., Hydrogen postconditioning promotes survival of rat retinal ganglion cells against ischemia/reperfusion injury through the PI3K/Akt pathway. Biochem Biophys Res Commun, 2018. 495(4): p. 2462-2468.

Ogawa, H., et al., Prevention of ischemia-induced hearing loss by intravenous administration of hydrogen-rich saline in gerbil. Neurosci Lett, 2018. 665: p. 195-199.

Tao, Y., et al., The Comparative Efficiency of Intraperitoneal and Intravitreous Injection of Hydrogen Rich Saline against N-Methyl-N-Nitrosourea Induced Retinal Degeneration: A Topographic Study. Front Pharmacol, 2017. 8: p. 587.

Fransson, A.E., et al., Hydrogen Inhalation Protects against Ototoxicity Induced by Intravenous Cisplatin in the Guinea Pig. Front Cell Neurosci, 2017. 11: p. 280.

Chu, Y.Y., et al., [The protection of hydrogen-rich saline on a rat dry eye model induced by scopolamine hydrobromide]. Zhonghua Yan Ke Za Zhi, 2017. 53(5): p. 363-372.

Chen, L., et al., Molecular mechanisms underlying the protective effects of hydrogen-saturated saline on noise-induced hearing loss. Acta Otolaryngol, 2017: p. 1-6.

Cejka, C., et al., Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress. Oxid Med Cell Longev, 2017. 2017: p. 8906027.

Wang, R., et al., Postconditioning with inhaled hydrogen promotes survival of retinal ganglion cells in a rat model of retinal ischemia/reperfusion injury. Brain Res, 2016. 1632: p. 82-90.

Igarashi, T., et al., Hydrogen prevents corneal endothelial damage in phacoemulsification cataract surgery. Sci Rep, 2016. 6: p. 31190.

Chen, T., et al., Protective effects of hydrogen-rich saline against N-methyl-N-nitrosourea-induced photoreceptor degeneration. Exp Eye Res, 2016. 148: p. 65-73.

Yokota, T., et al., Protective effect of molecular hydrogen against oxidative stress caused by peroxynitrite derived from nitric oxide in rat retina. Clin Experiment Ophthalmol, 2015.

Qi, L.S., et al., Sirtuin Type 1 Mediates the Retinal Protective Effect of Hydrogen-Rich Saline Against Light-Induced Damage in Rats. Invest Ophthalmol Vis Sci, 2015. 56(13): p. 8268-79.

Sun, J.C., et al., Hydrogen-rich saline promotes survival of retinal ganglion cells in a rat model of optic nerve crush. PLoS One, 2014. 9(6): p. e99299.

Moossavi, A., F. Bagheri, and H.R. Farkhani, Capabilities of hydrogen Molecules for use in the prevention and treatment in noise induced hearing loss. Rehabilitation Medicine 2014. 2(4).

Kurioka, T., et al., Inhaled hydrogen gas therapy for prevention of noise-induced hearing loss through reducing reactive oxygen species. Neurosci Res, 2014.

Chen, L., et al., Hydrogen-Saturated Saline Protects Intensive Narrow Band Noise-Induced Hearing Loss in Guinea Pigs through an Antioxidant Effect. PLoS One, 2014. 9(6): p. e100774.

Yang, C.X., H. Yan, and T.B. Ding, Hydrogen saline prevents selenite-induced cataract in rats. Molecular Vision, 2013. 19: p. 1684-93.

Tian, L., et al., Hydrogen-rich saline ameliorates the retina against light-induced damage in rats. Med Gas Res, 2013. 3(1): p. 19.

Zhou, Y., et al., Hydrogen-rich saline alleviates experimental noise-induced hearing loss in guinea pigs. Neuroscience, 2012. 209: p. 47-53.

Xiao, X., et al., Protective effects of hydrogen saline on diabetic retinopathy in a streptozotocin-induced diabetic rat model. Journal of Ocular Pharmacology and Therapeutics, 2012. 28(1): p. 76-82.

Song, D., et al., Hydrogenrich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats. Mol Med Rep, 2018. 18(2): p. 1925-1938.

Matsuoka, H., et al., Hydrogen gas improves left ventricular hypertrophy in Dahl rat of salt-sensitive hypertension. Clin Exp Hypertens, 2018: p. 1-5

Feng, R., et al., Early Aerobic Exercise Combined with Hydrogen-Rich Saline as Preconditioning Protects Myocardial Injury Induced by Acute Myocardial Infarction in Rats. Appl Biochem Biotechnol, 2018.

Chi, J., et al., Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats. Frontiers in Physiology, 2018. 9: p. 1026.

Chen, K., et al., Hydrogen-rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus-activated kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncol Lett, 2018. 16(1): p. 167-178.

Zalesak, M., et al., Molecular hydrogen potentiates beneficial anti-infarct effect of hypoxic postconditioning in isolated rat hearts: a novel cardioprotective intervention. Can J Physiol Pharmacol, 2017. 95(8): p. 888-893.

Yang, J., et al., Hydrogen-containing saline alleviates pressure overload-induced interstitial fibrosis and cardiac dysfunction in rats. Mol Med Rep, 2017. 16(2): p. 1771-1778.

Tamura, T., et al., Efficacy of inhaled HYdrogen on neurological outcome following BRain Ischemia During post-cardiac arrest care (HYBRID II trial): study protocol for a randomized controlled trial. Trials, 2017. 18(1): p. 488.

Ridwan, R.D., W.S. Juliastuti, and R.D. Setijanto, Effect of electrolyzed reduced water on Wistar rats with chronic periodontitis on malondialdehyde levels. Dental Journal (Majalah Kedokteran Gigi), 2017. 50(1): p. 10-13.

Katsumata, Y., et al., The Effects of Hydrogen Gas Inhalation on Adverse Left Ventricular Remodeling After Percutaneous Coronary Intervention for ST-Elevated Myocardial Infarction- First Pilot Study in Humans. Circ J, 2017.

Gao, Y., et al., Hydrogen Gas Attenuates Myocardial Ischemia Reperfusion Injury Independent of Postconditioning in Rats by Attenuating Endoplasmic Reticulum Stress-Induced Autophagy. Cell Physiol Biochem, 2017. 43(4): p. 1503-1514.

Gao, Y., et al., Hydrogen-rich saline attenuates hippocampus endoplasmic reticulum stress after cardiac arrest in rats. Neurosci Lett, 2017. 640: p. 29-36.

Chen, O., et al., High-concentration hydrogen protects mouse heart against ischemia/reperfusion injury through activation of thePI3K/Akt1 pathway. Sci Rep, 2017. 7(1): p. 14871.

Zhang, Y., et al., Hydrogen (H2) Inhibits Isoproterenol-Induced Cardiac Hypertrophy via Antioxidative Pathways. Front Pharmacol, 2016. 7: p. 392.

Wang, P., et al., Hydrogen Inhalation is Superior to Mild Hypothermia in Improving Cardiac Function and Neurological Outcome in an Asphyxial Cardiac Arrest Model of Rats. Shock, 2016. 46(3): p. 312-8.

Gao, Y., et al., Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis. Mediators Inflamm, 2016. 2016: p. 1320365.

Zhang, G., et al., Pharmacological postconditioning with lactic Acid and hydrogen rich saline alleviates myocardial reperfusion injury in rats. Sci Rep, 2015. 5: p. 9858.

Yu, Y., et al., Protective effects of hydrogen-rich medium on lipopolysaccharide-induced monocytic adhesion and vascular endothelial permeability through regulation of vascular endothelial cadherin. Genet Mol Res, 2015. 14(2): p. 6202-12.

Wu, F., et al., Treatment with hydrogen molecule attenuates cardiac dysfunction in streptozotocin-induced diabetic mice. Cardiovasc Pathol, 2015. 24(5): p. 294-303.

Song, G., et al., Molecular hydrogen stabilizes atherosclerotic plaque in low-density lipoprotein receptor-knockout mice. Free Radic Biol Med, 2015. 87: p. 58-68.

06 Human Studies

Zhu, Q., et al., Positive effects of hydrogen-water bathing in patients of psoriasis and parapsoriasis en plaques. Sci Rep, 2018. 8(1): p. 8051.

Nishimaki, K., et al., Effects of Molecular Hydrogen Assessed by an Animal Model and a Randomized Clinical Study on Mild Cognitive Impairment. Curr Alzheimer Res, 2018. 15(5): p. 482-492.

Nakayama, M., et al., Novel haemodialysis (HD) treatment employing molecular hydrogen (H2)-enriched dialysis solution improves prognosis of chronic dialysis patients: A prospective observational study. Sci Rep, 2018. 8(1): p. 254.

Ostojic, Sergej M., et al. “28-days Hydrogen-rich Water Supplementation Affects Exercise Capacity in Mid-age Overweight Women: 2942 Board# 225 June 1 3.” Medicine & Science in Sports & Exercise 50.5S (2018): 728-729.

Yang, Q., et al., Protective effect of hydrogen-rich water on liver function of colorectal cancer patients treated with mFOLFOX6 chemotherapy. Mol Clin Oncol, 2017. 7(5): p. 891-896.

Trivic, T., et al., Drinking hydrogen-rich water for 4 weeks positively affects serum antioxidant enzymes in healthy men: a pilot study. Current Topics in Nutraceutical Research, 2017. 15(1): p. 45-48.

Sun, Y.P. and L. Sun, Selective protective effect of hydrogen water on free radical injury of athletes after high-intensity exercise. Biomedical Research on Trace Elements, 2017. 28(10): p. 4558-4561.

Shibayama, Y., et al., Hydrogen-rich Water Modulates Redox Status Repeated Three Consecutive Days Of Strenuous Exercise (3298 Board# 203 June 2). Medicine & Science in Sports & Exercise, 2017. 49(5s): p. 941.

Ono, H., et al., Hydrogen Gas Inhalation Treatment in Acute Cerebral Infarction: A Randomized Controlled Clinical Study on Safety and Neuroprotection. J Stroke Cerebrovasc Dis, 2017.

Nishimaki, K., et al., Effects of molecular hydrogen assessed by an animal model and a randomized clinical study on mild cognitive impairment. Curr Alzheimer Res, 2017.

Mizuno, K., et al., Hydrogen-rich water for improvements of mood, anxiety, and autonomic nerve function in daily life. Med Gas Res, 2017. 7(4): p. 247-255.

Korovljev, D., et al., Molecular hydrogen affects body composition, metabolic profiles, and mitochondrial function in middle-aged overweight women. Ir J Med Sci, 2017.

Katsumata, Y., et al., The Effects of Hydrogen Gas Inhalation on Adverse Left Ventricular Remodeling After Percutaneous Coronary Intervention for ST-Elevated Myocardial Infarction- First Pilot Study in Humans. Circ J, 2017.

A. Ponte, A., et al., Effects of hydrogen rich water on prolonged intermittent exercise. J Sports Med Phys Fitness, 2017.

Tamasawa, A., et al., Hydrogen gas production is associated with reduced interleukin-1beta mRNA in peripheral blood after a single dose of acarbose in Japanese type 2 diabetic patients. Eur J Pharmacol, 2015. 762: p. 96-101.

Qian, L.R. and J.L. Shen, Successful treatment with hydrogen rich water in a case of chronic graft-versus-host-disease. Med Gas Res, 2016. 6(3): p. 177-179.

Maeda, K., et al., Improvement of the fraction of human mercaptalbumin on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid: a prospective observational study. Renal Replacement Therapy, 2016. 2(1): p. 42.

Kawamura, T., et al., Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness. Jpn J Phys Fitness Sports Med,, 2016. 65(3): p. 297-305.

Drid, P., et al., Molecular Hydrogen Affected Post-Exercise Recovery in Judo Athletes: 3820 Board# 259 June 4, 9: 30 AM-11: 00 AM.”. Medicine and science in sports and exercise, 2016. 48(5): p. 1071.

Tanikawa, R., et al., Relationship between Exhaled Hydrogen and Human Neutrophil Function in the Japanese General Population. Hirosaki Medical Journal, 2015. 65: p. 138-146.

Nakayama, M., et al., Novel haemodialysis (HD) treatment employing molecular hydrogen (H2)-enriched dialysis solution improves prognosis of chronic dialysis patients: A prospective observational study. Sci Rep, 2018. 8(1): p. 254.

Hosgood, S.A., et al., Hydrogen Gas Does Not Ameliorate Renal Ischemia Reperfusion Injury in a Preclinical Model. Artif Organs, 2018.

Cheng, T.C., et al., Nephroprotective effect of electrolyzed reduced water against cisplatin-induced kidney toxicity and oxidative damage in mice. J Chin Med Assoc, 2018. 81(2): p. 119-126.

Xing, Z., et al., Hydrogen Rich Water Attenuates Renal Injury and Fibrosis by Regulation Transforming Growth Factor-beta Induced Sirt1. Biol Pharm Bull, 2017. 40(5): p. 610-615.

Nakayama, M., et al., Dissolved molecular hydrogen (H2) in Peritoneal Dialysis (PD) solutions preserves mesothelial cells and peritoneal membrane integrity. BMC Nephrol, 2017. 18(1): p. 327.

Chen, J., et al., Hydrogen-Rich Saline Alleviates Kidney Fibrosis Following AKI and Retains Klotho Expression. Front Pharmacol, 2017. 8: p. 499.

Maeda, K., et al., Improvement of the fraction of human mercaptalbumin on hemodialysis treatment using hydrogen-dissolved hemodialysis fluid: a prospective observational study. Renal Replacement Therapy, 2016. 2(1): p. 42.

Li, J., et al., Hydrogen-Rich Saline Promotes the Recovery of Renal Function after Ischemia/Reperfusion Injury in Rats via Anti-apoptosis and Anti-inflammation. Front Pharmacol, 2016. 7: p. 106.

Du, H., et al., Hydrogen-Rich Saline Attenuates Acute Kidney Injury After Liver Transplantation via Activating p53-Mediated Autophagy. Transplantation, 2016. 100(3): p. 563-70.

Terawaki, H., et al., Successful treatment of encapsulating peritoneal sclerosis by hemodialysis and peritoneal lavage using dialysate containing dissolved hydrogen. Perit Dial Int, 2015. 35(1): p. 107-12.

Tange, Y., S. Takesawa, and S. Yoshitake, Dialysate with high dissolved hydrogen facilitates dissociation of indoxyl sulfate from albumin. Nephrourol Mon, 2015. 7(2): p. e26847.

Peng, Z., et al., Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice. Int J Clin Exp Pathol, 2015. 8(3): p. 2680-9.

Homma, K., et al., Inhalation of Hydrogen Gas Is Beneficial for Preventing Contrast-Induced Acute Kidney Injury in Rats. Nephron Exp Nephrol, 2015.

Guo, S.X., et al., Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation. J Transl Med, 2015. 13: p. 183.

Xin, H.G., et al., Consumption of hydrogen-rich water alleviates renal injury in spontaneous hypertensive rats. Mol Cell Biochem, 2014. 392(1-2): p. 117-24.

Terawaki, H., et al., Effect of a hydrogen (H2)-enriched solution on the albumin redox of hemodialysis patients. Hemodial Int, 2014. 18(2): p. 459-66.

Liu, W., et al., A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats. Int J Clin Exp Med, 2014. 7(4): p. 919-26.

Gu, H., et al., Pretreatment with hydrogen-rich saline reduces the damage caused by glycerol-induced rhabdomyolysis and acute kidney injury in rats. J Surg Res, 2014. 188(1): p. 243-9.

Zhu, W.J., et al., Amelioration of cardio-renal injury with aging in dahl salt-sensitive rats by H2-enriched electrolyzed water. Med Gas Res, 2013. 3(1): p. 26.

Kato, S., et al., Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity. J Nanosci Nanotechnol, 2012. 12(5): p. 4019-27.

Li, H., et al., Hydrogen-rich saline protects against small-scale liver ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress. Life Sci, 2018. 194: p. 7-14.

Ishikawa, T., et al., Post-reperfusion hydrogen gas treatment ameliorates ischemia reperfusion injury in rat livers from donors after cardiac death: a preliminary study. Surg Today, 2018.

Chen, M., et al., Hydrogen protects against liver injury during CO2 pneumoperitoneum in rats. Oncotarget, 2018. 9(2): p. 2631-2645.

Zhai, X., et al., Hydrogen-rich saline improves nonalcoholic fatty liver disease by alleviating oxidative stress and activating hepatic PPARalpha and PPARgamma. Mol Med Rep, 2017. 15(3): p. 1305-1312.

Wang, D., et al., The protective role of hydrogen-rich saline against liver injury caused by acetaminophen in mice. Int J Clin Exp Med, 2017. 10(8): p. 11646-11654.

Uto, K., et al., Hydrogen Rich Solution Attenuates Cold Ischemia-Reperfusion Injury in Rat Liver Transplantation. Transplantation, 2017. 101(5S-3): p. S18.

Lin, C.P., et al., Anti-oxidant and anti-inflammatory effects of hydrogen-rich water alleviate ethanol-induced fatty liver in mice. World J Gastroenterol, 2017. 23(27): p. 4920-4934.

Li, H., et al., Inhalation of high concentrations of hydrogen ameliorates liver ischemia/reperfusion injury through A2A receptor mediated PI3K-Akt pathway. Biochem Pharmacol, 2017. 130: p. 83-92.

Iketani, M., et al., Preadministration of Hydrogen-Rich Water Protects Against Lipopolysaccharide-Induced Sepsis and Attenuates Liver Injury. Shock, 2017. 48(1): p. 85-93.

Golshahi, H., et al., Protective effect of intraportal infusion of hypothermic hydrogen-rich saline solution on hepatic warm ischemia/reperfusion injury in rat model. Brazilian Journal of Veterinary Pathology, 2017. 10(1): p. 10-21.

Shimada, S., et al., Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver. Artif Organs, 2016. 40(12): p. 1128-1136.

Shi, Q., et al., Hydrogen-Rich Saline Attenuates Acute Hepatic Injury in Acute Necrotizing Pancreatitis by Inhibiting Inflammation and Apoptosis, Involving JNK and p38 Mitogen-Activated Protein Kinase-dependent Reactive Oxygen Species. Pancreas, 2016. 45(10): p. 1424-1431.

Liu, Q., et al., Hydrogen-rich saline protects against mitochondrial dysfunction and apoptosis in mice with obstructive jaundice. Mol Med Rep, 2016. 13(4): p. 3588-96.

Zhang, J.Y., et al., Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. World J Gastroenterol, 2015. 21(14): p. 4195-209.

Zhang, C.B., et al., Hydrogen gas inhalation protects against liver ischemia/reperfusion injury by activating the NF-κB signaling pathway. Experimental and Therapeutic Medicine, 2015. 9(6): p. 2114-2120.

Yu, J., et al., Molecular hydrogen attenuates hypoxia/reoxygenation injury of intrahepatic cholangiocytes by activating Nrf2 expression. Toxicol Lett, 2015. 238(3): p. 11-19.

Sobue, S., et al., Simultaneous oral and inhalational intake of molecular hydrogen additively suppresses signaling pathways in rodents. Mol Cell Biochem, 2015. 403(1-2): p. 231-41.

Lee, P.C., et al., Concomitant inhibition of oxidative stress and angiogenesis by chronic hydrogen-rich saline and N-acetylcysteine treatments improves systemic, splanchnic and hepatic hemodynamics of cirrhotic rats. Hepatol Res, 2015. 45(5): p. 578-88.

Tan, Y.C., et al., Hydrogen-rich saline attenuates postoperative liver failure after major hepatectomy in rats. Clin Res Hepatol Gastroenterol, 2014. 38(3): p. 337-45.

Matsuno, N., et al., Beneficial effects of hydrogen gas on porcine liver reperfusion injury with use of total vascular exclusion and active venous bypass. Transplant Proc, 2014. 46(4): p. 1104-6.